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Assessing blood cell adhesion to cultured endothelial cells.
Provide more physiologically relevant environment compared to protein functionalized assays.
Any type of primary human endothelial cells can be cultured under physiologic flow and activated via disease-specific pro-inflammatory agents.
Cultured endothelial cells can be activated via patients’ own blood and/or blood products.
Whole blood or isolated RBCs, WBCs, and platelets.
Relevant Literature AJH, 2018 BJH, 2020
1.https://curesickle.org