Assessing blood cell adhesion to cultured endothelial cells.
About
Mimicking in vivo conditions
Provide more physiologically relevant environment compared to protein functionalized assays.
Endothelial cell type and source
Any type of primary human endothelial cells can be cultured under physiologic flow and activated via disease-specific pro-inflammatory agents.
Patient specific activation
Cultured endothelial cells can be activated via patients’ own blood and/or blood products.
Sample
Whole blood or isolated RBCs, WBCs, and platelets.
TypicalUses
Clinical Assay, Personalized Medicine, Research, Drug Development
Targeted Therapies
Drugs that target RBC sickling/deformability (e.g., Voxelotor) in SCD.
Curative Therapies
NHLBI-supported multisite study to assess RBC mediated microcapillary occlusions before and after a gene-based therapy for curative intent(1).
References
Relevant Literature
AJH, 2018
BJH, 2020
1.https://curesickle.org