Single cell adhesion analysis of red blood cells and white blood cells in SCD
About
Microchannel Functionalization
- Laminin
- Fibronectin
- ICAM-1
- VCAM-1
- E-selectin
- P-selectin
Physiological Relevance
Micro-physiologic flow conditions, shear stress range from 1 dyne/cm2 to 10 dyne/cm2.
Sample
Use of whole blood in EDTA, Nacitrate, or heparin, with no preprocessing or dilution.
TypicalUses
Clinical Assay, Personalized Medicine, Research, Drug Development
Targeted Therapies
Drugs that target RBC sickling/deformability (e.g., Voxelotor)in SCD
Curative Therapies
NHLBI-supported multisite study to assess RBC mediated microcapillary occlusions before and after a gene-based(2) therapy for curative intent(1)
References
Publications:
Blood Advances, 2020;
AJH, 2018 & 2020, Translational Research,
2016;
Scientific Reports, 2014
1. https://pubmed.ncbi.nlm.nih.gov/32208292/
2. https://curesickle.org